Light microscopy summer school (7th edition)
Registration for the summer school is now closed, but you're still welcome to join us for the Open Lecture days on 19 & 20 May.
We're thrilled to invite you to the 7th edition of the Light Microscopy Summer School, a hands-on, week-long training program designed to explore the microscopic world in stunning detail.
Whether you're a PhD student or postdoc, this course offers a friendly and practical environment to sharpen your imaging skills and discover the latest in light microscopy. From the basics of optics to cutting-edge techniques like super-resolution and functional imaging, we'll guide you through it all. Each day combines interactive lectures with hands-on sessions, so you won't just learn the theory, you'll get to apply it right away using advanced equipment and real biological samples.
What to expect:
Objectives:
Required skills:
The basics of light microscopy: history of microscopy and basics of optics
Diffraction, conjugated planes, and Köhler illumination
Conjugated planes and Köhler Illumination
Contrast and imaging methods, phase contrast (PH), and differential interference contrast (DIC)
Setting up phase contrast and how to keep a microscope clean
What is fluorescence, fluorescence microscopy, and immunohistochemistry
Choosing filters for fluorescence imaging
Basics of confocal imaging, 3Dimaging
Live cell imaging, spinning disc, and 2-Photon microscopy
Improving microscopy resolution beyond the diffraction limit: deconvolution and superresolution techniques
Functional microscopy: "The F- techniques (FRAP/ FRET/ FLIM)"
Introduction to spectral unmixing and fluorescence confocal imaging in plants
Quenching/ removal of autofluorescence and bleaching techniques
Introduction to spatial imaging
Connected volumetric imaging
Fluorescent probes, labelling and delivery strategies
Viventis SCAPE; #ESCAPE the limits of 3D live cell imaging
Correlative light and electron microscopy
Imaging large samples and light-sheet microscopy
High-Speed, Large-Volume 3D Imaging with the Ultramicroscope Blaze
Image processing and code of conduct of image analysis
Accessible AI ecosystems enabling microscopy-driven biomedical discoveries
Advanced Fiji