On the 10th and 11th of December, the 5th edition of the Next-Generation Synthetic Biology Conference will take place in Ghent, Belgium. Earlier editions of this conference had a strong focus on Genome Engineering, while the upcoming conference will put the spotlight on Synthetic Biology. In this double interview with Prof. Tom Ellis (Imperial College London) and Prof. Sven Eyckerman (VIB-UGent Center for Medical Biotechnology), two members of the organizing committee, we deep dive into the world of Synthetic Biology, present research, and future perspectives.