Asset 2 Conferences

From 3D Light to 3D Electron Microscopy

19-22 March 2024, Ghent, Belgium
This event is sold-out
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From 3D Light to 3D Electron Microscopy

From 3D Light to 3D Electron Microscopy
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This event on Correlative Light & Electron Microscopy (CLEM) is jointly organized by ZEISS, The Francis Crick Institute, EMBL and VIB.

Imaging life in 3 dimensions and in its most natural form – when imaging biological samples with both light and electron microscopy, the main goal is to elucidate biological processes and their ultrastructural context in three dimensions. In most recent years, technology has pushed the resolution limits in light microscopy and volume-EM methods were named one of 7 technologies to watch by Nature magazine.

When using correlative, or multimodal microscopy, a large variety of different imaging techniques can be combined. New and improved workflows combining two, or more imaging modalities are enabling more detailed insights into biological processes than ever before.

The datasets generated by these new workflows are increasingly large and complex. New developments in data management not only focus on image processing and visualization of these large datasets, but also on i.e. file compatibility, big data handling and storage.

This event will combine scientific sessions with a broad range of workshops where correlative workflows, tools, tips & tricks will be shared between participants and trainers/speakers.

Host City: Ghent
Want to know more about the host city of this conference? Find out where the best restaurants, bars, or last-minute print shops are. Check the city page here.


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Confirmed speakers

Program

Day 1 - Tuesday, 19 March, 2024

Address: Oude Vismijn - Sint-Veerleplein 4, 9000 Gent (See map)

15:30
16:30
Registration & coffee
16:30
17:00
Welcome by Anneke Kremer
17:00
18:30
Keynote session

Session chair: Erin Tranfield

17:00
17:45
Enabling New Insights into the World of Plants via 3D and Correlative Approaches
Keynote speaker
17:45
18:30
Form follows function – Correlative volumeEM studies of organelles
18:30
22:00
Dinner @ Oude Vismijn

Additional fee

Day 2 - Wednesday, 20 March, 2024

Address: Technologiepark-Zwijnaarde 71, 9052 Gent

08:00 Bus Transfer (More info)

08:20
08:50
Welcome coffee
08:50
09:00
Welcome
09:00
12:45
Session 1: CLEM Workflows

Session chair: Paolo Ronchi

09:00
09:30
Correlative Organelle Microscopy: Functional characterization of organelles by correlative live-cell and volume electron microscopy
09:30
09:45
ATUM-Tomo bridges scales by combining array tomographywith electron tomography

Martina Schifferer

German Center for Neurodegenerative Diseases, DE; Cluster for Systems Neurology, DE
Selected from abstracts
09:45
10:00
Finding and imaging intracellular components with single molecule fluroescence guided cryo-electron tomography

Mart Last

Leiden University Medical Centre, NL
Selected from abstracts
10:00
10:30
Coffee break
10:30
11:00
Unveiling novel functions and cell types at the underexplored blood-CSF brain barrier using 3D electron microscopy
12:45
14:00
Lunch & Poster session
16:00
17:15
Session 2: Imaging Technologies

Session chair: Saskia Lippens

16:00
16:30
Hard X-ray Imaging of Soft Biological Tissue: Is it more than simply a step linking 3D Light with 3D Electron Microscopy?
16:30
17:00
Correlative electron and ion microscopy for metabolic imaging
17:00
17:15
Femtosecond laser, the swiss knife of CLvEM

Perrine Bomme

Institut Pasteur, FR
Selected from abstracts
17:15
17:30
break

Day 3 - Thursday, 21 March, 2024

Address: Technologiepark-Zwijnaarde 71, 9052 Gent

08:00 Bus transfer (More info)

08:30
09:00
Welcome coffee
09:00
11:00
Session 3: Diverse Biological Samples

Session chair: Lucy Collinson

09:00
09:30
MICOS and Mitochondrial Dysfunction in Human Health Explored Using 3D EM Techniques
09:30
09:45
Targeted volume electron microscopy of P. falciparum infected RBC - endothelial interactions in a 3D blood brain barrier model of cerebral malaria

Hannah Fleckenstein

EMBL Barcelona, ES; EMBL Heidelberg, DE
Selected from abstracts
09:45
10:00
A volume of volumes: serial section electron tomography of entire malaria parasites

Irina Bregy

Radboud University, NL
Selected from abstracts
10:00
10:30
Coffee break
10:30
11:00
Implementation of CLEM approaches for plant tissues
12:45
14:00
Lunch & Poster session 2
15:55
17:35
Session 4: Data Management / Handling

Session chair: Yannick Schwab

15:55
16:25
Expanding the "data toolkit" in vEM
16:25
16:55
A modular strategy for segmentation, spatial analysis, and visualization of volume electron microscopy datasets
16:55
17:10
Improving correlative light and electron microscopy image registration using blood vessel alignment

Yentl Van den Berghe

VIB-UGent Center for Inflammation Research, BE
Selected from abstracts
17:10
17:25
EMDiffuse: a diffusion-based deep learning method augmenting ultrastructural imaging and volume electron microscopy

Chixiang Lu

The Univerisity of Hong Kong, HK
Selected from abstracts
17:25
17:35
Break
19:00
20:00
Get together @ Technologiepark

This network reception is included in your registration.

20:00
Bus transfer

Day 4 - Friday, 22 March, 2024

Address: Technologiepark-Zwijnaarde 71, 9052 Gent

08:00 Bus transfer (More info)

08:30
09:00
Welcome coffee
09:00
12:00
Session 5: Molecular Tools

Session chair: Anneke Kremer

09:00
09:30
Labels and label-free approaches for live-cell to ultrastructure examination and identification
09:30
09:45
Oxygenation gradients in multicellular spheroids: are they always as expected?

Angela Debruyne

Ghent University, BE
Selected from abstracts
09:45
10:15
The role of the phosphatidylserine neighbourhood in apoptotic cell clearance
10:15
10:30
Volume Electron Microscopy with the 64-beams FAST-EM

Arent Kievits

Department of Imaging Physics, Delft University of Technology, NL
Selected from abstracts
10:30
11:00
Coffee break
11:00
11:45
Exploring life across scales with vCLEM
11:45
12:00
Closing words
12:15
Bus transfer

Organizing committee

Abstracts

Poster information
The format for your poster should be: A0 (841 x 1189 mm / 33.1 x 46.8 in), portrait orientation.

If you're not registered before 20 February 2024, your abstract will be excluded from the abstract list and you will not have the chance to present your work at the conference.

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The full list of abstracts will be available on 05-03-2024 until 22-06-2024
This list will only be available for registered attendees of the event.

Attendees

The full list of attendees will be available on 05-03-2024 until 22-06-2024
This list will only be available for registered attendees of the event.